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Fujifilm Wako, Japan) with 15% FBS (Biosera, USA), non-essential amino acids (Thermo Fisher Scientific, USA), and 1.15 mM 1-thioglycerol in a humidified atmosphere of 5% CO 2 at 37 C, as previously described 62
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The body tightly regulates B12 absorption through intrinsic factor, a protein produced in the stomach that binds to B12 and facilitates its uptake in the terminal ileum
