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Description
A time-course study with at least 3-5 time points is required to capture the trajectory

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The homogenates were centrifuged at 20,400 g for 20 min at 4 C, and the resulting supernatants were appropriately diluted and used in the assays, which were conducted according to the manufacturers instructions
Mental Health Issues: Prolonged lack of sleep can contribute to the development or worsening of mental health conditions
