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Description
Heart tissues isolated from FLAG-BNIP3 mice were initially washed with PBS, dissected, and homogenised in immunoprecipitation (IP) lysis buffer composed of 1 Cell Lysis Buffer (Cell Signaling), 100 mM PMSF (Roche), and 1 protease and phosphatase inhibitor cocktail (ThermoFisher)

Some anti-aging practitioners recommend repeating this cycle biannually

After weighing, fresh thalli were immediately frozen in liquid nitrogen

Some studies have confirmed that GPX4 and cystine/glutamate transporter system are downstream targets of Nrf2 (Dodson et al., 2019)
