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Elution (pH elution, ionic strength elution, competitive elution, reduced polarity of eluent, chaotropic eluents, elution gradient, flow rates) Troubleshooting: (Data were taken from Affinity Chromatography Principles and Methods, 18-1022-29) 1

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The alterations in glutamine metabolism in tumor cells are an important outcome of the changes in the energy metabolism of tumor cells

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Dysregulation of these macronutrients can lead to oxidative stress, liver disease, and even mortality [11, 12]

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10.1016/j.jfca.2011.01.008

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