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Figure 5 In order to determine if the phosphorylation of Ser42, Ser184, and Tyr198 was involved in the effect of GSTP on HMGB1, six GSTP mutants were constructed, including GSTP-S42A (a Ser42 non-phosphorylatable mutant), GSTP-S42D (a Ser42 constant-phosphomimetic mutant), GSTP-S184A (a Ser184 non-phosphorylatable mutant), GSTP-S184D (a Ser184 constant-phosphomimetic mutant), GSTP-Y198F (a Tyr198 non-phosphorylatable mutant), as well as GSTP-Y198D (a Tyr198 constant-phosphomimetic mutant) (Figure S4B in Supplementary Material)

This explains the difference in growth rates of cells when moved from the in vitro setting to mice, where extracellular GSH and cysteine is limited in vivo

Early-life smoking, cardiovascular disease risk, and the mediating role of DNA methylation biomarkers of aging

Hepatology 66 , 908921 (2017)