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doi: 10.1111/ane.12830 66

Erythrocytes were rinsed 3 times with cold 0.9% NaCl solution and hemolyzed by the addition of a 9-fold volume of cold phosphate-buffered saline (PBS) (50 mM, pH 7.4)
Evidence also exists that methylation memories in DNA are involved in maintaining gene expression patterns

Zeigler DR
