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The laser settings were standardized across all patients, with energy set to 120 mJ, density at 3%, and frequency at 250 Hz
Nervous System Regulation: We've addressed chronic stress, HPA axis dysregulation, and autonomic nervous system imbalance
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Finally, the membranes were washed with PBST three times, and a ChemiDoc XRS System (Bio-Rad, CA, USA) was used to detect the expression of proteins with an enhanced chemiluminescence (ECL) kit (NCM Biotech, Suzhou, China)
