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The method according to claim 1, wherein the detection of the optical purity of L-3-cyano -2 - hydroxypropyl trimethyl ammonium and the content of its D-isomer in step (3) includes the following steps:( 1 ) Preparation of the derivatization reagent solution: D-type or L-type optical pure compound of formula (II) is dissolved in solvent to prepare a 0.01 100 mg / ml solution under the dark conditions;( 2 ) Preparation of the test solution: Proper amount of L-3-cyano -2 - hydroxypropyl trimethyl ammonium sample is hydrolyzed with hydrochloric acid and then the aqua ammonia is added in to adjust the PH value until it is neutral;(3) Preparation of the control solution: Proper amount of racemic carnitine is used to prepare the control solution;( 4 ) Derivatization reaction: Proper amount of optically pure derived reagent of formula (II) is mixed with L-carnitine (or D-carnitine) test solution and carnitine control solution

Mooli and Ramakrishnan, 2022b)

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