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This cream significantly and rapidly reduced skin pigmentation and was equally effective as 4% hydroquinone [136, 195]
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The resulting pEarleyGate 202/ 35S::FLAG-GSNOR1 vector was transformed into Agrobacterium tumefaciens strain GV3101(pMP90), which was subsequently used to transform WT plants by floral dipping 62
The lysate was cleared by centrifugation and the protein was purified by Ni-His-affinity chromatography in batch mode
