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Western blot Cells were lysed in 1 Laemmli buffer and centrifuged at 10,000 g for 2 min through a Nucleo Spin filter (Machery Nagel)
2012 doi: 10.1097/MAJ.0b013e3182579dc6

This can result in patients using an incorrect, often higher, dose due to an error in measurement relating to milligrams or markings on the syringe
