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Through examination of MCF-7 cells, the investigation delineated the concerted influence of SFN, derived from cruciferous vegetables, and HDAC inhibitors like Trichostatin A (TSA) on gene expression patterns associated with 1,25(OH)2D3 activity
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Immunofluorescence staining The tumor-bearing liver tissues from C57BL/6 mice were frozen section, and then the slices were permeabilized with 0.3% Triton X-100 solution for 15 min at room temperature
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