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Consistent with the biological changes, immunoblotting analysis showed the late induction of p-H2AX and H2AX [45], suppression of ataxia telangiectasia mutated (ATM) [46] and pS1981-ATM, pT68-Chk2 (checkpoint kinase 2 serine/threonine kinase) [47], rap1 interacting factor (RIF)1 [48], Cdc25C [49], and Cyclin D1, and the induction of p21, CDK1, pS133-Cyclin B1 and Cyclin B1 (Fig

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