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In addition, the overexpression of LRG1 is observed to promote epithelialmesenchymal transition (EMT) by activating MAPK/p38 signaling in thyroid carcinoma cells (Zhang et al., 2016)

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Tris 20 mM, NaCl 150 mM, Tween 20 detergent 0.1% (w/v)) for 1 h at room temperature, followed by primary antibody incubation in TBST overnight at 4 C

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