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Briefly, human iPSCs were reprogramed from wild-type human foreskin fibroblasts (ATCC, catalog: CRL-2522) 44,45 using a non-integrating Sendai virus (CytoTune-iPS 2.0 Sendai Reprogramming Kit, Thermo Fisher Scientific, catalog: A16517) and maintained in StemFlex Medium (Thermo Fisher Scientific, catalog: A3349401) with medium changes every other day 46

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Importantly, atfs-1(null);nhr-180(cmh19) also exhibited increased body bending/thrashing rate compared to atfs-1(null) worms