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Western blot analysis was performed with proteins from liver tissues (60 g) using anti-STAT3 (1:1000 dilution), phosphorylated STAT3 (1:1000 dilution) (Cell Signaling, Beverly, MA), and PPAR antibodies (Abcam, Cambridge, UK)

263 Individuals with social anxiety disorder were found to have lower omega-3 fatty acid levels in their red blood cell membranes compared with healthy people, 264 and in a study in depressed patients, those with anxiety had lower blood plasma levels of EPA and DHA than those without anxiety

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