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1:500, mouse clone MRPm5, Abcam, Cambridge, UK), anti-breast cancer resistance protein (1:500, mouse clone BXP-21, Santa Cruz Biotechnology Inc.), anti-C/EBP- (1:500, rabbit polyclonal, # sc150, Santa Cruz Biotechnology Inc.), anti-CHOP (1:500, mouse monoclonal, #ab11419, Abcam), anti-TRB3 (1:500, rabbit polyclonal, #133001-AP, Proteintech, Chicago, IL), anti-caspase-3 (1:1000, mouse clone C33, GeneTex, Hsinhu City, Taiwan), anti-CRT (rabbit polyclonal #PA3900, Affinity Bioreagents, Rockford, IL), anti-NOS I (1:500, mouse clone 16, BD Biosciences, Franklin Lakes, NJ), anti-NOS II (1:1000, mouse clone 4E5, ThermoFisher Scientific Inc.), anti-NOS III (1:500, mouse clone 3, BD Biosciences), anti-pancadherin (1:500, goat clone C-19, Santa Cruz Biotechnology Inc.), anti--tubulin (1:1000, mouse clone D10, Santa Cruz Biotechnology Inc.), followed by the horseradish peroxidase-conjugated secondary antibodies (Bio-Rad)

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Convenient Administration: Lipo-C injections are administered intramuscularly, making them a convenient option for individuals seeking to enhance their weight management efforts without the need for daily pills or supplements

The filtrate is subjected to alkaline saponification to liberate bound L-carnitine in the solution