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The homogenates were centrifuged at 15,000 rpm for 10 min, and the protein concentration in the supernatant was determined using the Pierce Bicinchoninic Acid assay Kit (Thermo Scientific)
Consequently, studies have focused on how NAD + precursors impact the biodiversity and composition of the gut microbiome and the resulting effects of this

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Folate and neural tube defects: The role of supplements and food fortification
