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For anaerobic growth, experiments were typically set up under ambient conditions and subsequently incubated statically at 37 C inside an anaerobic chamber (Coy Laboratory Products), except for the RNA-seq time course, RT-qPCR, and proteomics experiments, which were not only incubated anaerobically but also set up under anaerobic conditions given the relatively short experimental timeframe

Although cystine starvation has been shown to induce oxidative stress, we did not observe any increase in soluble or lipid ROS production in cystine-starved cells (Fig

By supporting the skins natural repair mechanisms and strengthening the skin barrier function, GHK-Cu may help reduce the severity and duration of inflammatory acne lesions

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