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Short-chain fatty acids (SCFAs), produced through the fermentation of dietary fibers by beneficial microbes, are key players in immune modulation
Subsequent restriction digest of PCR fragments with ApaI resulted in 141 bp and 516 bp fragments when positive for the F973 modification, whereas nonmodified WT DNA did not contain the ApaI site and thus still showed a 658 bp band upon ApaI digestion
Hauss DJ
