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The studys methodology aimed to elucidate the mechanisms underlying hesperidins antiviral activity

Cell viability was assessed using the CCK-8 assay (Dojindo, Japan), and optical density (OD) was measured at 450 nm using a VARIOSKAN LUX reader (Thermo Fisher Scientific, USA)

10.1038/nrmicro1557 71

Patients typically present with an acute 3-4 g/dl drop in hemoglobin along with dark urine due to hemoglobinuria, elevated lactate dehydrogenase, and elevated reticulocyte count two to four days following an offending stressor
