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Despite the improvement of some surrogate biomarkers such as proinflammatory cytokines, antioxidative capacities, and lipid or glycemic profiles, the positive trends in clinical outcomes were less promising with single supplementation

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Then, the offspring were sacrificed and various brain tissues, including the amygdala, hypothalamus and hippocampus, were isolated, flash frozen in dry ice, and then stored in a 80C freezer for analysis of gene expression and oxidative stress

Each body is unique, so the duration of the effects of a vitamin B12 shot may vary