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It has also been observed in vitro that SCs and neurites prefer to travel towards softer stiffness substrates 35,36 , suggesting that materials within the lumen which are stiffer than the surrounding tissues can be inhibitory to nerve growth

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For patients whose levels fall in the borderline or gray zone, a methylmalonic acid (MMA) test provides greater specificity MMA levels rise when B12 is functionally inadequate at the cellular level, even if serum B12 appears borderline normal

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