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Nonetheless, this process heavily relies on the ability to isolate and separate the somatic cell population from the germ cell population prior to cultivation, a task currently constrained by the absence of a specific SSC marker [76]

Several factors influence the choice of needle and syringe: Type of injection : Intramuscular (IM) or subcutaneous (SC) Gauge of the needle : Diameter of the needle Length of the needle : Determines the depth of injection Volume of the syringe : Determines the amount of B12 to be injected 3