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Let it trickle down the glass wall toward the powder rather than jetting directly onto the lyophilized material

Ongoing research and clinical trials continue to explore their therapeutic potential in improving long-term outcomes for stroke survivors

The membrane was first blocked in 5% (wt./vol.) skim milk powder (Lab M Limited, Heywood, UK) in tris-buffered saline with 0.1% tween20 (TBST) for 1 h at RT, then was probed with the primary antibody (concentrations and manufacturers in Supplementary Table 4) diluted in TBST with 5% BSA for 16 h at 4 C on a rocker

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