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Antibodies The following primary antibodies were used: anti-GPX4 (#52455), anti-SLC7A11 (#12691), anti-phosphoSirT1 (Ser47) (#2314), anti-phosphoCDK2 (Thr160) (#2561), anti-PhosphoRB1 S807/811 (#8516), anti-cleaved PARP (#5625), anti-cleaved Casp3 (#9664), anti- Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (#4370), Anti- 5-Lipoxygenase (ALOX5) (#3289) from Cell Signaling Technology at a 1:1000 dilution

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To assess levels of global DNA methylation, both fusion proteins are constructed using the DNA-binding domain of a methyl-CpG-binding domain protein such as MBD2, which has a 100-fold preference for methylated CpG sites over unmethylated CpG sites

Research Use Notice All products in this category are supplied exclusively for laboratory research purposes