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Where indicated, lysates were incubated additionally with DTT (10100 mM, Sigma) for 1 h at 37 C before electrophoresis

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Could we explore if a B12 supplement might help my symptoms?" This approach moves the conversation away from "fixing a number" and toward "optimising how you feel." GPs are often much more receptive to private results when they are presented alongside a clear history of symptoms and a responsible, collaborative attitude
