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Cytokine and chemokine assays Protein was extracted using a 2-cm piece of distal colon and homogenized in 300 l of Meso Scale Discovery lysis buffer with protease and phosphatase inhibitors as described in the assay protocol
Pharmacological management of obesity: an Endocrine Society clinical practice guideline
The Baltimore Hospital is acknowledged as the first to develop vitamin cocktails that can be injected or administered intravenously
Finally, the control group received no topical treatment
