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Additionally, the PciI-to-PciI fragment (1.4 kb) spanning the entire vpu and N-terminal half of the env coding sequence was deleted and replaced with an 8-nt linker carrying a BssHII site, ensuing that neither Vpu nor functional Env is produced in this construct
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doi: 10.1016/j.jep.2023.117089 96 ChyauC-CWangH-FZhangW-JChenC-CHuangS-HChangC-Cet al
