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[DOI] [PubMed] [Google Scholar] 111.Mubarakshina M.M., Ivanov B.N

washing buffer: 10 mM TrisHCl, 154 mM NaCl, 1 mM CaCl 2 , 0.05% Tween 20, pH 7.4

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The biosynthesis of glutathione necessitates the availability of glutamate, glycine, and cysteine, with cysteine being particularly crucial due to its redox-active thiol group (R-SH) (23, 24)