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Real-time PCR was performed using an SYBR Green I technology and MxPro- Mx3000P QPCR machine (Stratagene, CA, USA), and a master mix was prepared with Smart Quant Green Master Mix with dUTP & ROX Kit (Protech, Taipei, Taiwan)

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Once reconstituted, the BPC-157 solution should be stored in a refrigerator at 2-8C and used within a specified timeframe, typically a few weeks, to ensure its bioactivity

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