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The impact of AFB1 on the gut microbiota was evaluated by extracting the contents of the broiler chickens duodenum, following AFB1 exposure and using 16S rRNA sequencing to analyze the changes in abundance and diversity of the gut microbiota (Huang et al., 2023)

In both groups, significant increases in cobalamin occurred at 28 and 90 day intervals

The cultured neurons were maintained with complete culture medium composed of B27 supplement (Gibco, USA), L-glutamine (Life Technologies), and Neurobasal-A medium (DMEM/F12) (Gibco, USA) at 37 C in a 7% CO 2 incubator for 7 days to ensure the growth of nerve synapses