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L-lactate levels were normalized to protein levels for each well individually

Hao et al., 2017b) and the highest concentration of SCFAs in fish intestine is in the posterior region

Furthermore, autophagic defects lead to impaired ferritin degradation, and the release of lysosomal iron ions exacerbates ferroptosis, forming a vicious cycle of "autophagic dysfunction-iron overload-lipid peroxidation"

Off-target detection To determine the site-specific cleavage of the CRISPRCas9 system in vivo, the potential off-target loci were searched using Cas-OFFinder ( The selected potential off-target sites were initially PCR-amplified and then subjected to a DNA sequencing assay