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Molecular assessments revealed significant alterations in gene expression, with heat stress upregulating pro-apoptotic Bax and Caspase-3 and HSP 70 (Hspa1a/b) while downregulating Cx43 (Gja1), confirming germ cell apoptosis and loss of Sertoli-Leydig intercellular communication

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Similar proinflammatory effects along with increased susceptibility to influenza-A virus was seen in vivo wherein mice exposed to acrolein for 4 days exhibited an accelerated inflammatory response with higher macrophages and neutrophils, and greater IL-1, IL-1, IL-6, TNF, IFN-, KC, and MCP-1 in the bronchial fluid