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After fatty acid treatment, the organoids were incubated with 3.8 M BODIPY 493/503 (Life Technologies) for 30 min in culture medium at 37 C and then washed two times with PBS before being replaced with fresh culture medium containing DRAQ5 (Thermo Fisher) at 1:1500

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The PDE5 inhibitory constituents of medicinal plant extracts include alkaloids, phenolics, and polycyclic aromatic hydrocarbons