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Enzyme-linked immunosorbent assay (ELISA) Spinal cord tissue and muscle tissue were weighted and quickly homogenized in ice cold PBS according to manufactures protocols followed by centrifugation at 4500 g for 5 min at 4 C

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The "TwoSampleMR" R package in the R software was utilized to extract IVs associated with exposure by setting parameters (P = 1e-5, clump = True, r2 = 0.001, kb = 10000) [23, 29]

Quantitative PCR (qPCR) assay The mouse liver tissues were pulverized
