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Plasma metabolomics for amino acids and pyruvate Amino acids were extracted from 20 L of plasma with 200 L of methanol containing Try-d8 (1600ng), Phe-d8 (160ng), Tyr-d4 (160ng), Leu-d3 (320ng), Ile-13C6,15N (160ng), Met-d3 (160ng), Val-d8 (400ng), Glu-d3 (2400ng), Thr-13C4 (320ng), Ser-d3 (400ng), Asp-d3 (3200ng), Ala-d4 (1600ng), Asn d3,15N2(3200ng), Gly-d2 (16000ng), Gln-13C5 (3200ng), Pro-d7 (800ng), Cit-d4 (80ng), His 13C6 (400ng), Arg-13C6 (1600ng), Orn-13C5 (400ng), Lys-d8 (400ng), and pyruvic acid-13C5 (80 ng) as the internal standards for Try, Phe, Tyr, Leu, Ile, Met, Val, Glu, Thr, Ser, Asp, Ala, Asn, Gly, Gln, Pro, Cit, His, Arg, Orn, Lys, and pyruvic acid (Pyr) respectively

passage6), G-CSF (200400 g/kg b.wt.), Dihexa (24 mg/kg b.wt.) and/or Vehicle were administered to male Lewis rats locally via hydrogel at the site of nerve repair, systemically (i.v./i.p), and/or to gastrocnemius muscle

Instead, numerous small molecule inhibitors exert their anti-cancer effects through off-target interactions, with their anti-tumor activity either independent of, or partially reliant on, their intended targets [6,7,8]

Laboratory digestion process of breastmilk, blood and urine To measure mercury levels, 0.5 mL of each sample were placed in a glass flask with 5 mL of nitric acid [HNO 3 ] for overnight reaction