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Synthesized RNAs were purified using MicroSpin G-25 Columns (GE Healthcare Life Sciences), quantified by Nanodrop (Invitrogen), and verified on a 5% TBE urea gel stained with SYBR Gold (Life Technologies) for 20 min prior to imaging using the ChemiDoc Imaging system (Bio-Rad Laboratories)

Overall, luteolin represents a multi-target pharmacological candidate with translational potential in gynecologic and endocrine disorders, warranting further optimization and early-phase clinical investigation

Hesperidin can enhance insulin signaling by promoting the phosphorylation of insulin receptor substrate (IRS) proteins and Akt kinase

Onset of effects typically occurs within 4 to 6 weeks