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Description
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cerevisiae ubiquitin (250 M), mUbe1 (800 nM), Ubc2 (2 M), Ubr1 (800 nM), and ATP (10 mM) for 60 min at 30C in ubiquitination buffer (50 mM Tris pH 8.0, 150 mM NaCl, 10 mM MgCl 2 , 1 mM DTT)

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