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In total, 50 L of the diluted Matrigel gel was added to the upper chamber of the transwell, gently tilted to ensure even spreading, and incubated at 37 C for 4 h in a cell culture incubator

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The first, larger, outer capsule is coated with a special acid-resistant enteric coating, so when swallowed it bypasses the stomach and is resistant to its degrading factors such as digestive enzymes and stomach acids