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Vascular endothelial growth factor (VEGF), a typical marker of angiogenesis during wound healing, was quantitatively assessed via ELISA on day 3, revealing a significant increase in the G/D-CuP group (Fig
Explants were incubated overnight at 4C with mouse monoclonal antibody against III-tubulin (Millipore, MAB5564) diluted 1:10,000 in blocking solution
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