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Given previous reports of atypical social and aggressive behaviors in KI mice [17, 22], which could potentially modulate the aggression provoked in CD1 mice and thus confound SDS exposure, we quantified the number of attacks sustained by each experimental subject

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If the experiment cannot be denatured, you can only try to change the upstream conditions, either increase the length of his to increase the probability of tag exposure, or modify the position of the His tag