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19,20 The increase in mucus production, and the decrease in CFTR activity can precede COPD and persist once disease is established, 21 indicating that the presence of oxidant stress plays a role in the early onset of disease and is an attractive therapeutic target

Of note, such metabolomic alterations can be observed in the R group 12 months after the acute disease

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Finally, blood samples were collected after sacrificing to determine oxidative stress biomarkers: nitrite (NO 2 ), superoxide anion radical (O 2 ), hydrogen peroxide (H 2 O 2 ), the index of lipid peroxidation (TBARS), reduced glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD)