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Protein extracts were then subjected to SDSPAGE and transferred to Immobilin-FL polyvinylidene fluoride transfer membrane

We calibrate and validate the model with experimental data from control C3H mice and from the rd1 mouse model for the disease RP 37

To detect LYSET, M6P (scFv M6P), GNPT3HA KI, or the uninduced 10xGCN4, the anti-protein A HRP (PA00-03, Rockland, for LYSET and M6P) or mouse HRP (115-035-046, Jackson labs, for GNPT3HA KI) secondary antibodies were used at 1:10,000 dilution

In conditions of oxidative stress, melatonin reduces the expression of activated caspase-3 and the Bax/Bcl-2 ratio, preventing neuronal apoptosis [50,99,100,101]