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In addition, we use filter-tips or filter plates to remove the flow-through after binding the phosphopeptides to the beads, to wash the beads, and elute the phosphopeptides using centrifugation or a vacuum manifold, respectively

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This is consistent with our analysis which showed a statistically significant positive association of infiltration of CD8 + T cells, CD4 + T cells, macrophages, neutrophils, and dendritic cells with TGFB1 expression