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shRNA knockdown of Bmi-1 reveals a critical role for p21-Rb pathway in NSC self-renewal during development

The treatments are conducted via the administration of Botox (Onabotulinumtoxin A) at a dilution of 100 U in 4 cc of 0.9% saline solution (concentration 2.5 U/0.1 ml) using a 1 cc syringe with a 30 Gauge needle

Curcumin exerts antioxidant effects through multiple pathways, directly scavenging reactive oxygen species and reactive nitrogen species, reducing oxidative damage to cellular components, with its chemical structure, including two phenolic rings connected by a beta-diketone moiety, enabling it to neutralize free radicals and inhibit lipid peroxidation (Alisi et al., 2018