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Control culture variables that affect signaling: Passage number, confluence, serum concentration, extracellular matrix coating, oxygen condition, mycoplasma status, baseline cell health, and image-analysis thresholds can all influence FAK phosphorylation, VEGFR2 readouts, and tube-formation results

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Myth: Its okay to use the same needle to draw and inject. Fact: While technically possible, many people use a larger needle to draw the water and a much smaller, thinner needle for the actual injection to maximize comfort

Chang and colleagues showed in 2011 that the peptide upregulates growth-hormone receptor expression on tendon fibroblasts and increases their migration and survival in culture, an effect that has been reproduced across several Achilles- and quadriceps-tendon transection models in rats