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Description
It was demonstrated in animal studies that unabsorbed quaternary amines such as choline, phosphatidylcholine, betaine or l-carnitine can be metabolized by gut microorganisms to produce the intermediate compound trimethylamine (TMA)

These signals are believed to be sent when the body is experiencing a reduced and low-calorie diet

Thereafter, 90 min was allowed for recovery of muscle from insertion trauma

The PCR product for each gene was amplified using Phusion polymerase (Thermofisher) and specific primers (see detailed sequence in Appendix Table S5)
