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For experiments involving oxygen control, purified CD8 + T cells were immediately acclimatized to the required oxygen tension (21%, 5% or 1% O 2 ) for 2 h in complete RPMI 1640 supplemented with 10% FBS, 100 U ml 1 penicillin and 100 g ml 1 streptomycin, before activation with anti-CD3/CD28 Dynabeads and 30 U ml 1 IL-2
Nonetheless, an isotope tracer analysis found that only serine, not glycine, feeds 1C metabolism in cancer cells in vitro [48]
This presents a challenge for those who follow a vegan and vegetarian way of eating, placing them at risk of Vitamin B12 deficiency

A healthy gut barrier is essential for overall wellness
