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Oct 15 2019;9(1):14804

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The identification of a constitutive NADPH-independent cytosolic disulfide reductase system in mammals Based on the well-established requirement for the NADPH-dependent disulfide reductase systems in microbial models, as discussed above, it seemed to be a foregone conclusion that mammalian cells would also require the presence of at least one of the two NADPH-dependent cytosolic disulfide reductases for survival

Our generation of pure GSS-CAM-TPP enabled us to assess whether this impacted our approach by quantifying any conversion of GSS-CAM-TPP to its corresponding thioether GS-CAM-TPP in the presence of TCEP (Supplementary Fig