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On the contrary, physiological or homeostatic level of ROS is involved in the activation of intracellular pathways responsible for spermatozoa maturation, capacitation, hyperactivation, acrosomal reaction, chemotactic processes, formation of the mitochondrial capsule and condensation of sperm DNA as well as fusion with the female gamete to ensure fertilization
Within the acidified endosomes, TLR9 directly binds these CpG-rich mtDNA fragments, initiating an innate immune response
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